5UKW
Crystal structure of human Glucose 6-phosphate Dehydrogenase mutant (A277C) complexed with G6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-19 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.97643 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 59.099, 177.817, 216.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.230 - 2.650 |
| R-factor | 0.19101 |
| Rwork | 0.189 |
| R-free | 0.23368 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bh9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.644 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.230 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.154 | 1.532 |
| Rpim | 0.050 | |
| Number of reflections | 16889 | |
| <I/σ(I)> | 17.2 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 8.8 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 100 mM Tris-HCl pH 9, 200 mM MgCl2, 13% PEG 4000 and 20% glycerol |
