5UKI
Mn2+ and Zn2+ requirements for the lariat debranching enzyme, Dbr1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1, 1.28, 1.30 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.341, 70.920, 109.266 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.880 - 1.800 |
| R-factor | 0.17393 |
| Rwork | 0.172 |
| R-free | 0.21027 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.788 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.529 |
| Number of reflections | 35775 | 413 |
| <I/σ(I)> | 34.1 | 3.81 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 24% PEG 8000, 0.1 M HEPES, pH 7.5, 10% glycerol |
