5UKG
Crystal Structure of the genetically encoded calcium indicator K-GECO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.284, 129.249, 78.715 |
| Unit cell angles | 90.00, 106.27, 90.00 |
Refinement procedure
| Resolution | 75.560 - 2.360 |
| R-factor | 0.20976 |
| Rwork | 0.206 |
| R-free | 0.27473 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 75.560 |
| High resolution limit [Å] | 2.360 |
| Number of reflections | 30298 |
| <I/σ(I)> | 5.1 |
| Completeness [%] | 94.6 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5,000 |
