5UK7
Escherichia coli Hfq bound to dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 65.749, 65.795, 81.996 |
Unit cell angles | 105.93, 92.28, 119.92 |
Refinement procedure
Resolution | 25.685 - 3.000 |
R-factor | 0.2129 |
Rwork | 0.210 |
R-free | 0.25980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gib |
RMSD bond length | 0.006 |
RMSD bond angle | 1.131 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
Rmerge | 0.106 | 0.029 | 0.350 |
Number of reflections | 18997 | ||
<I/σ(I)> | 9.3 | ||
Completeness [%] | 83.8 | 95.3 | 53.1 |
Redundancy | 1.8 | 1.9 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 28-38% MPD, 0.1 M Tris pH 7.5-8.5 |