5UJ6

Crystal Structure of Bacteroides Uniformis beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033200
Spacegroup name	P 21 21 21
Unit cell lengths	74.728, 142.943, 181.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.624 - 1.900
R-factor	0.144
Rwork	0.143
R-free	0.18020
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	1.276
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.624	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.068	0.448
Number of reflections	152573
<I/σ(I)>	21.1	5
Completeness [%]	99.4	98.7
Redundancy	10.9	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% PEG 3350, 0.2 M Potassium Chloride