5UIN
X-ray structure of the W305A variant of the FdtF N-formyltransferase from salmonella enteric O60
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-29 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.467, 59.457, 107.599 |
Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.200 |
R-factor | 0.19078 |
Rwork | 0.188 |
R-free | 0.25221 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uij |
RMSD bond length | 0.011 |
RMSD bond angle | 1.874 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.095 | 0.264 |
Number of reflections | 45968 | 5534 |
<I/σ(I)> | 9.1 | 2.8 |
Completeness [%] | 98.7 | 96.6 |
Redundancy | 4.1 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MOPS 13-16% PEG 3350 200 mL LiCl 5 mM TDP 5 mM folinic acid |