5UIF
Crystal Structure of Native Ps01740
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.722, 58.902, 84.759 |
| Unit cell angles | 90.00, 110.37, 90.00 |
Refinement procedure
| Resolution | 46.480 - 2.570 |
| R-factor | 0.2218 |
| Rwork | 0.219 |
| R-free | 0.27160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n4g |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.464 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.480 |
| High resolution limit [Å] | 2.570 |
| Number of reflections | 11402 |
| <I/σ(I)> | 7.9 |
| Completeness [%] | 96.0 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18% PEG 8000 0.1 M CHES pH 9.0 |
