5UHP
Crystal structure of the core catalytic domain of human O-GlcNAcase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 89.722, 91.531, 94.517 |
| Unit cell angles | 77.27, 62.81, 63.03 |
Refinement procedure
| Resolution | 40.780 - 2.790 |
| R-factor | 0.242 |
| Rwork | 0.239 |
| R-free | 0.29200 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.780 |
| High resolution limit [Å] | 2.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M K Na tartrate tetrahydrate, 20% PEG 3350 |
