5UGW
STRUCTURE OF THE HUMAN TELOMERASE THUMB DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-18 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 92.425, 92.425, 50.178 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.680 - 2.310 |
| R-factor | 0.19781 |
| Rwork | 0.197 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.186 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.680 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 10654 | 813 |
| <I/σ(I)> | 18.68 | 2.19 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 10.8 | 10.25 |
| CC(1/2) | 0.999 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 50 mM Tris 8.5 0.8 M LiSo4 10 mM GSH/GSSG 3% 1,6-hexanediol |
