5UGN
DNA polymerase beta imidodiphosphate reactant complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-07 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.778, 79.922, 55.321 |
Unit cell angles | 90.00, 107.67, 90.00 |
Refinement procedure
Resolution | 23.154 - 1.997 |
R-factor | 0.1841 |
Rwork | 0.181 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fms |
RMSD bond length | 0.007 |
RMSD bond angle | 0.871 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.154 | 2.030 |
High resolution limit [Å] | 1.997 | 2.000 |
Rmerge | 0.074 | 0.504 |
Number of reflections | 28404 | 1317 |
<I/σ(I)> | 20.2 | 2.2 |
Completeness [%] | 99.2 | 92.4 |
Redundancy | 5.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350 |