Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.379, 84.057, 41.472 |
| Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
| Resolution | 28.890 - 1.900 |
| R-factor | 0.2206 |
| Rwork | 0.218 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.890 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.900 | 3.990 | 1.850 |
| Rmerge | 0.077 | 0.032 | 0.997 |
| Number of reflections | 18032 | ||
| <I/σ(I)> | 16 | 49 | 1 |
| Completeness [%] | 99.4 | 98.5 | 96.5 |
| Redundancy | 3.5 | 3.6 | 3 |
| CC(1/2) | 0.997 | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | complex of HIF2-B*:ARNT-B* 10-12 mg/mL in 10 mM Tris-HCl, pH8.0 and 50 mM KCl; Crystallization buffer of 50 mM Bis-Tris, pH5.4 and 16% PEG3350. |
