5UFK
Structure of the effector protein SidK (lpg0968) from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-14 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.7712 |
| Spacegroup name | P 43 |
| Unit cell lengths | 52.960, 52.960, 127.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.905 - 2.300 |
| R-factor | 0.1744 |
| Rwork | 0.172 |
| R-free | 0.21230 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.496 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.096 | 1.060 |
| Number of reflections | 30728 | |
| <I/σ(I)> | 16.8 | 2.18 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 7.6 | 7.4 |
| CC(1/2) | 0.999 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M TRIS hydrochloride pH 8.5, 0.2 M lithium sulfate monohydrate and 30% (w/v) polyethylene glycol 4,000 |
