5UFF

Crystal Structure of Variable Lymphocyte Receptor (VLR) RBC36 with Fucose(alpha-1-2)Lactose bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97840
Spacegroup name	P 1 21 1
Unit cell lengths	39.969, 47.503, 57.276
Unit cell angles	90.00, 85.69, 90.00

Refinement procedure

Resolution	39.856 - 2.137
R-factor	0.2259
Rwork	0.223
R-free	0.28240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6j
RMSD bond length	0.007
RMSD bond angle	1.117
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.865	2.210
High resolution limit [Å]	2.137	2.137
Rpim	0.070	0.700
Number of reflections	11758
<I/σ(I)>	8
Completeness [%]	97.0
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	11.4 mg/mL RBC36, 6.9 mM fucose(alpha-1-2)lactose, 140 mM ammonium chloride, 22% PEG3350