5UFB

Crystal Structure of Variable Lymphocyte Receptor (VLR) Tn4-22 (Apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	I 41
Unit cell lengths	83.688, 83.688, 115.073
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.844 - 1.844
R-factor	0.2068
Rwork	0.206
R-free	0.21310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6j
RMSD bond length	0.007
RMSD bond angle	1.024
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.844	1.910
High resolution limit [Å]	1.844	1.844
Rpim	0.030	0.410
Number of reflections	33959
<I/σ(I)>	15.1
Completeness [%]	100.0
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	21.6 mg/mL Tn4-22, 100 mM sodium acetate, pH 4.6, 200 mM lithium sulfate, 2.7 M sodium chloride