5UF8
Crystal structure of the ARF family small GTPase ARF2 from Candida albicans in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-12-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.289, 64.362, 79.236 |
| Unit cell angles | 78.11, 83.73, 72.77 |
Refinement procedure
| Resolution | 24.873 - 1.872 |
| R-factor | 0.2364 |
| Rwork | 0.235 |
| R-free | 0.26980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mr3 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.647 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.054 | 0.409 |
| Number of reflections | 53906 | |
| <I/σ(I)> | 41.35 | 5.04 |
| Completeness [%] | 93.4 | 90.7 |
| Redundancy | 4.1 | 4.3 |
| CC(1/2) | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 30% PEG2K MME, 0.2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6, 5 mM magnesium chloride, 5 mM ADP |
