5UEW

BRD2 Bromodomain2 with A-1360579

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	35.996, 41.601, 45.756
Unit cell angles	67.40, 66.90, 74.85

Refinement procedure

Resolution	18.270 - 1.830
R-factor	0.201
Rwork	0.199
R-free	0.23800
RMSD bond length	0.010
RMSD bond angle	0.970
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.900
High resolution limit [Å]	1.800
Rmerge	0.056
Number of reflections	18605
<I/σ(I)>	6.5
Completeness [%]	94.8
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0