5UEL

Crystal structure of 354NC102 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.218, 82.499, 117.211
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.910 - 1.600
R-factor	0.177
Rwork	0.177
R-free	0.20130
RMSD bond length	0.007
RMSD bond angle	1.145
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	38.910
High resolution limit [Å]	1.600
Rmerge	0.108
Number of reflections	77546
<I/σ(I)>	11.12
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.1 M sodium acetate trihydrate pH 4.6, 2.0 M ammonium sulfate