5UEG

RNA primer-template complex with guanosine dinucleotide G(5')pppp(5')G ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	99
Detector technology	CCD
Collection date	2016-10-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.995
Spacegroup name	P 3
Unit cell lengths	48.416, 48.416, 81.965
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.21197
Rwork	0.208
R-free	0.30148
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dhc
RMSD bond length	0.018
RMSD bond angle	3.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.100	0.554
Number of reflections	6425
<I/σ(I)>	15.14	1.26
Completeness [%]	96.6	74.8
Redundancy	5.1	3.6
CC(1/2)	0.978	0.916

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.05 M Magnesium chloride, 1.2 M Lithium sulfate, 50 mM MES pH 6.5, 2 mM Cobalt (II) chloride