5UED
RNA primer-template complex with guanosine dinucleotide ligand G(5')pp(5')G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2016-07-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.997 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 43.911, 43.911, 85.406 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.21147 |
| Rwork | 0.210 |
| R-free | 0.23216 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dhc |
| RMSD bond length | 0.025 |
| RMSD bond angle | 3.216 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.079 |
| Number of reflections | 15113 |
| <I/σ(I)> | 28.4 |
| Completeness [%] | 95.1 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.05 M magnesium chloride, 0.2 M Ammonium citrate tribasic pH 7.0, 20% w/v Polyethylene glycol 3,350 |
