5UEA
Structure of antigen-Fab complex with Histone chaperone ASF1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97900 |
Spacegroup name | P 1 |
Unit cell lengths | 52.561, 81.770, 82.150 |
Unit cell angles | 70.51, 77.28, 81.67 |
Refinement procedure
Resolution | 39.347 - 1.701 |
R-factor | 0.1566 |
Rwork | 0.154 |
R-free | 0.20730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.852 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.347 |
High resolution limit [Å] | 1.701 |
Number of reflections | 133679 |
<I/σ(I)> | 8.1 |
Completeness [%] | 97.0 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, .2 M Ammonium chloride |