5UE9

WT DHODB with orotate bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2012-08-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	80.207, 151.911, 214.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.389 - 2.720
R-factor	0.198
Rwork	0.195
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ep2
RMSD bond length	0.009
RMSD bond angle	1.214
Data reduction software	iMOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_2621)

Data quality characteristics

	Overall
Low resolution limit [Å]	53.000
High resolution limit [Å]	2.720
Number of reflections	34552
<I/σ(I)>	7.34
Completeness [%]	99.0
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	298	0.1M NaAcetate pH 4, PEG 4k 12% w/v, glycerol 3% v/v