5UDZ
Human LIN28A in complex with let-7f-1 microRNA pre-element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-08-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 |
Unit cell lengths | 76.429, 76.429, 105.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.520 - 2.000 |
R-factor | 0.1659 |
Rwork | 0.164 |
R-free | 0.20030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ts2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.277 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.520 | 2.071 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.091 | 0.619 |
Number of reflections | 40547 | |
<I/σ(I)> | 10.79 | 1.73 |
Completeness [%] | 99.0 | 96.96 |
Redundancy | 2.7 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.1 M Citrate, pH 8.0 |