5UD8

Crystal Structure of Mutant Ig-like Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	23.790, 62.530, 125.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.250 - 1.800
R-factor	0.2079
Rwork	0.206
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.097
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.250	44.250	1.900
High resolution limit [Å]	1.800	5.690	1.800
Rmerge	0.127	0.090	0.351
Number of reflections	17947
<I/σ(I)>	9.2
Completeness [%]	98.7	99.7	93.6
Redundancy	5.8	6	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	293	30% PEG 6000, 0.1 M Tris 8.0