5UCS
Class II fructose-1,6-bisphosphate aldolase E149A variant of Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-27 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.056, 82.826, 91.095 |
Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
Resolution | 29.936 - 1.408 |
R-factor | 0.165 |
Rwork | 0.164 |
R-free | 0.18750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c4u |
RMSD bond length | 0.008 |
RMSD bond angle | 0.875 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.936 |
High resolution limit [Å] | 1.408 |
Rmerge | 0.078 |
Number of reflections | 108617 |
<I/σ(I)> | 8.89 |
Completeness [%] | 65.9 |
Redundancy | 3.8 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | PEG 8000, PEG 1000, Calcium acetate, Tris-Acetic acid buffer |