5UCB

Structure of antigen-Fab complex with engineered switch residue region.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	193
Detector technology	PIXEL
Collection date	2015-06-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.033
Spacegroup name	P 1
Unit cell lengths	45.378, 49.180, 84.707
Unit cell angles	106.29, 95.71, 100.13

Refinement procedure

Resolution	35.589 - 1.521
R-factor	0.1396
Rwork	0.138
R-free	0.16880
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.589
High resolution limit [Å]	1.521
Number of reflections	100518
<I/σ(I)>	32.2
Completeness [%]	95.8
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	18% PEG3350,