5UBL
A circularly permuted version of PvdQ (cpPvdQ)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 271 |
| Detector technology | PIXEL |
| Collection date | 2016-06-15 |
| Detector | DECTRIS PILATUS3 X 1M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 121.251, 166.120, 94.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.990 - 1.800 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.20180 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.936 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 67.900 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 88115 |
| <I/σ(I)> | 8 |
| Completeness [%] | 98.0 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | 0.05 M Hepes (pH 7.5), 80 mM RbCl, 10% PEG 4000 |
