5UAZ

Crystal structure of the yeast nucleoporin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	41.497, 100.487, 85.225
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.282 - 1.753
R-factor	0.1585
Rwork	0.156
R-free	0.19880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3p3d
RMSD bond length	0.013
RMSD bond angle	1.250
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.282	1.816
High resolution limit [Å]	1.753	1.753
Rmerge	0.119
Number of reflections	18275
<I/σ(I)>	21.66	2.55
Completeness [%]	99.8	98.72
Redundancy	13.3	9.9
CC(1/2)		0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	20% (wt/vol) PEG 3350 0.2 M Ammonium sulfate