5UAI

Crystal structure of Methionyl-tRNA formyltransferase from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-17
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 21 21 2
Unit cell lengths	113.920, 109.060, 109.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.084 - 2.750
R-factor	0.1709
Rwork	0.167
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fmt
RMSD bond length	0.008
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.084	49.084	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.105	0.028	0.531
Number of reflections	36266
<I/σ(I)>	18.88	36.43	4.36
Completeness [%]	99.9	96	100
Redundancy	7.402	5.432	7.546
CC(1/2)	0.998	0.999	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	20.7 mg/mL PsaeA.17389.a.B1.PW37587 crystallized in sitting drop vapor diffusion with Rigaku MCSG1 B5: 0.2 M MgCl2; 0.1 M Tris pH 8.5; 25% PEG3350, cryo: 20% EG. tray 259534b5, puck rzw4-7