5UAG

Escherichia coli RNA polymerase mutant - RpoB D516V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-11
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97790
Spacegroup name	P 21 21 21
Unit cell lengths	184.826, 205.043, 307.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.992 - 3.399
R-factor	0.2771
Rwork	0.277
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yg2
RMSD bond length	0.011
RMSD bond angle	1.663
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.400
Number of reflections	144355
<I/σ(I)>	9.7
Completeness [%]	90.5
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	0.1 M HEPES-HCl (pH 6.7), 0.2 M Ca Acetate, 30% PEG400, 10 mM DTT