5UAD

MET Tyrosine Kinase Inhibition Enhances the Antitumor Efficacy of an HGF Antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9764848
Spacegroup name	P 21 21 21
Unit cell lengths	42.612, 47.468, 161.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.250
R-factor	0.1987
Rwork	0.196
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.175
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.290
High resolution limit [Å]	2.250	6.100	2.250
Rmerge	0.096	0.039	0.524
Total number of observations	70139
Number of reflections	15939
<I/σ(I)>	7.7
Completeness [%]	98.0	97	87.4
Redundancy	4.4	4.4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	273	19.% PEG 3350, 12.% Isopropanol, 0.1M HEPES pH 7.2