5U9L

Crystal structure of the intermembrane space region of the plastid division protein PARC6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	44.533, 124.546, 130.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.910 - 2.516
R-factor	0.1851
Rwork	0.181
R-free	0.25940
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.600
High resolution limit [Å]	2.500	5.200	2.500
Rmerge	0.139	0.095	0.907
Number of reflections	12543
<I/σ(I)>	6.2
Completeness [%]	99.5	99.1	98.4
Redundancy	8.3	8.9	7
CC(1/2)		0.996	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	18% polyethylene glycol (PEG) 4000, 0.1 M Magnesium acetate, 0.1 M Na-MES (pH 6.5), 0.05 M Potassium nitrate and 5% glycerol