5U93
Structure of the Regulatory Domain of the AraC Family Transcriptional Activator RhaR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.793, 100.793, 95.696 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.863 - 1.999 |
| R-factor | 0.1724 |
| Rwork | 0.171 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.084 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 1.999 | 2.050 |
| Rmerge | 0.034 | 0.203 |
| Number of reflections | 33840 | |
| <I/σ(I)> | 33.128 | 3.96 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 9.2 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100 mM MES pH 6.0, 50 mM Ca(OAc)2, 12 % PEG 3350 |
