5U91
Crystal structure of Tre/loxLTR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.260, 193.390, 89.020 |
| Unit cell angles | 90.00, 111.25, 90.00 |
Refinement procedure
| Resolution | 82.967 - 3.104 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q3u |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.639 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 96.690 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 71563 |
| <I/σ(I)> | 3.9 |
| Completeness [%] | 90.7 |
| Redundancy | 4.2 |
| CC(1/2) | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 10 mM sodium citrate, 25-32.5 % Peg 6000 |
