5U8M
A novel family of redox sensors in the streptococci evolved from two-component response regulators
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.330, 74.832, 102.799 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.368 - 2.105 |
R-factor | 0.1907 |
Rwork | 0.188 |
R-free | 0.23090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5u8k |
RMSD bond length | 0.011 |
RMSD bond angle | 1.050 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2148: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.071 | 0.586 |
Number of reflections | 64031 | |
<I/σ(I)> | 25.2 | 2.5 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 6.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.3 M Magnesium sulfate, 10% PEG 3350 |