5U5G
Psf3 in complex with NADP+ and 2-OPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.076, 87.369, 164.011 |
Unit cell angles | 90.00, 92.04, 90.00 |
Refinement procedure
Resolution | 46.324 - 2.048 |
R-factor | 0.1716 |
Rwork | 0.170 |
R-free | 0.20680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.957 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.581 | |
Number of reflections | 78862 | |
<I/σ(I)> | 9.6 | 2 |
Completeness [%] | 96.6 | 96.6 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 12 mg/mL Psf3, 43% v/v polypropylene glycol P400, 0.1 M Bis-Tris Methane pH 6.5 |