5U51

Structure of Francisella tularensis heterodimeric SspA (MglA-SspA) in complex with ppGpp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	57.620, 111.560, 141.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.610 - 2.800
R-factor	0.2151
Rwork	0.212
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pur 1yy7
RMSD bond length	0.003
RMSD bond angle	0.526
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.610	2.950
High resolution limit [Å]	2.800	2.800
Rmerge		0.874
Rpim		0.404
Number of reflections	23247
<I/σ(I)>	7.8	2
Completeness [%]	100.0	100
Redundancy	5.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	TRIS pH 8.5, PEG3350, ppGpp, magnesium chloride