5U4Y
IgG Fc bound to 3 helix of the B-domain from Protein A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 1996-12-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.950, 152.260, 46.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.075 - 2.499 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fc1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.962 |
| Data reduction software | HKL-2000 (1.3.0) |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.590 |
| High resolution limit [Å] | 2.499 | 2.499 |
| Rmerge | 0.285 | |
| Number of reflections | 25214 | |
| <I/σ(I)> | 30.4 | 3.9 |
| Completeness [%] | 97.1 | 99.1 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 25% PEG 3400, 0.1M magnesium acetate, 0.1 M HEPES pH 7.0. Protein concentration 5mg/mL. |
