5U4T

Crystal structure of a methyltransferase involved in the biosynthesis of gentamicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	293
Detector technology	PIXEL
Collection date	2015-06-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	2.38
Spacegroup name	P 21 21 21
Unit cell lengths	66.620, 66.782, 69.123
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.028 - 2.086
R-factor	0.1542
Rwork	0.151
R-free	0.21810
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	48.028
High resolution limit [Å]	2.086
Number of reflections	17433
<I/σ(I)>	24.15
Completeness [%]	92.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Magnesium chloride, tris pH 8, peg 3350 and peg 400