5U2J
MORF double PHD finger (DPF) in complex with histone H3K14bu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2016-06-21 |
Detector | NOIR-1 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.540, 69.190, 69.930 |
Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
Resolution | 34.693 - 1.600 |
R-factor | 0.1931 |
Rwork | 0.191 |
R-free | 0.23840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.904 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 34.700 |
High resolution limit [Å] | 1.600 |
Number of reflections | 40091 |
<I/σ(I)> | 12.1 |
Completeness [%] | 99.9 |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1 M sodium citrate tribasic, 0.1 M sodium hepes |