5U1Z
X-ray structure of the WlarG aminotransferase, apo form, from Campylobacter jejune
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-01 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 107.726, 56.837, 124.797 |
Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
Resolution | 29.970 - 1.600 |
R-factor | 0.15032 |
Rwork | 0.148 |
R-free | 0.18768 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3frk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.740 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.093 | 0.411 |
Number of reflections | 197128 | |
<I/σ(I)> | 8.4 | 2 |
Completeness [%] | 98.8 | 96.3 |
Redundancy | 4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MOPS (pH 7) 18-18% PEG-3350 |