5U1S
Crystal structure of the Saccharomyces cerevisiae separase-securin complex at 3.0 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 126.300, 126.300, 273.862 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.975 - 3.002 |
R-factor | 0.1928 |
Rwork | 0.190 |
R-free | 0.25050 |
Structure solution method | SIRAS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.650 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.144 | 0.058 | |
Rmeas | 0.140 | 0.060 | |
Rpim | 0.050 | 0.017 | 0.723 |
Total number of observations | 533070 | ||
Number of reflections | 51156 | ||
<I/σ(I)> | 6.3 | ||
Completeness [%] | 99.8 | 99.2 | 99.6 |
Redundancy | 10.4 | 12.3 | 7.6 |
CC(1/2) | 0.999 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | 0.25 M Na/K-phosphate (pH 6.0) and 14% (w/v) PEG 3350 |