5U0U
Crystal Structure of DH270.1 (unliganded, single-chain Fv) from the DH270 Broadly Neutralizing N332-glycan Dependent Lineage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.873, 111.284, 89.134 |
| Unit cell angles | 90.00, 120.46, 90.00 |
Refinement procedure
| Resolution | 49.851 - 3.428 |
| R-factor | 0.255 |
| Rwork | 0.254 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tqa |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.892 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.851 | 50.000 | 3.480 |
| High resolution limit [Å] | 3.420 | 9.260 | 3.420 |
| Rmerge | 0.184 | 0.089 | 0.652 |
| Rmeas | 0.233 | ||
| Rpim | 0.140 | ||
| Total number of observations | 32526 | ||
| Number of reflections | 14420 | ||
| <I/σ(I)> | 3.8 | ||
| Completeness [%] | 81.5 | 75.5 | 85.5 |
| Redundancy | 2.3 | 2.4 | 2.2 |
| CC(1/2) | 0.971 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 2.5 M sodium chloride, 100 mM sodium acetate, pH 4.5 and 200 mM LiSO4 |
