5U0N

Crystal structure of a methyltransferase in complex with the substrate involved in the biosynthesis of gentamicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	298
Detector technology	PIXEL
Collection date	2015-10-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.45
Spacegroup name	P 21 21 21
Unit cell lengths	57.975, 67.385, 69.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.538 - 2.115
R-factor	0.1691
Rwork	0.166
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u4t
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (phenix.refine: 1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.540
High resolution limit [Å]	2.115
Rmerge	0.220
Number of reflections	16081
<I/σ(I)>	10.53
Completeness [%]	99.3
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	magnesium chloride, tris pH 8,0, peg 3350, peg 400