5U0B
Structure of full-length Zika virus NS5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2016-09-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.500, 197.000, 95.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.775 - 3.000 |
| R-factor | 0.2327 |
| Rwork | 0.231 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k6m |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.511 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.775 | |
| High resolution limit [Å] | 3.000 | |
| Rmerge | 0.156 | 0.715 |
| Rpim | 0.103 | 0.475 |
| Number of reflections | 50661 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 277 | 1.0 M ammonium sulfate plus 1% PE G 3350, 0.1 M Bis-Tris, pH 5.5 |
