5TZM

59th Ig domain of human obscurin (OBSCN Ig59)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2014-04-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	60.980, 60.980, 47.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.490 - 1.177
R-factor	0.165
Rwork	0.164
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yz8
RMSD bond length	0.033
RMSD bond angle	1.459
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.490	1.219
High resolution limit [Å]	1.177	1.177
Rmerge	0.135	0.539
Number of reflections	33764
<I/σ(I)>	22.98	5.07
Completeness [%]	98.8	92.53
Redundancy	19.5	10.7
CC(1/2)	0.988	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	17% tacsimate, 0.1M HEPES pH 7.5, 4% PEG3350, 10 mg/mL C-terminal his-tagged Ig59