5TZG

Structure of the BldD CTD(D116A)-(c-di-GMP)2, form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-25
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 41 2 2
Unit cell lengths	82.098, 82.098, 59.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.421 - 2.500
R-factor	0.2025
Rwork	0.197
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tzd
RMSD bond length	0.007
RMSD bond angle	1.383
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.100	2.740
High resolution limit [Å]	2.500	2.500
Rmerge		0.158
Rpim	0.023
Number of reflections	13016
<I/σ(I)>	21.4	6.5
Completeness [%]	97.2	97.1
Redundancy	5.5	5.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	20% PEG 3000, 0.1 M sodium acetate pH 4.6, 0.1 M zinc chloride