5TZD

Structure of the WT S. venezulae BldD-(CTD-c-di-GMP)2 assembly intermediate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-06
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	108.300, 108.300, 49.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.888 - 1.749
R-factor	0.2151
Rwork	0.207
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5khd
RMSD bond length	0.024
RMSD bond angle	2.390
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.827	1.940
High resolution limit [Å]	1.749	1.749
Rmerge		0.112
Number of reflections	31775
<I/σ(I)>	17	5.1
Completeness [%]	94.7	79.5
Redundancy	4.1	2.1
CC(1/2)	0.997	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	30% PEG 1500, 100 mM sodium acetate, pH 5.0