5TYY

DNA Polymerase Mu Product Complex, Mn2+ (60 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-17
Detector	RAYONIX MX-225
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.985, 68.670, 110.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.082 - 1.931
R-factor	0.1874
Rwork	0.186
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.008
RMSD bond angle	0.958
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.700
High resolution limit [Å]	1.670	4.530	1.670
Rmerge	0.079	0.062	0.904
Rmeas	0.087
Rpim	0.034
Total number of observations	344374
Number of reflections	53715
<I/σ(I)>	10.6
Completeness [%]	99.8	98.9	99.1
Redundancy	6.4	6.2	5.6
CC(1/2)		0.994	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000