5TXV
HslU P21 cell with 4 hexamers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.497, 420.860, 176.418 |
| Unit cell angles | 90.00, 98.56, 90.00 |
Refinement procedure
| Resolution | 49.187 - 7.086 |
| R-factor | 0.276 |
| Rwork | 0.274 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hqy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.614 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 7.220 |
| High resolution limit [Å] | 7.086 | 18.940 | 7.100 |
| Rmerge | 0.105 | 0.054 | 0.694 |
| Number of reflections | 18657 | ||
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 98.9 | 97.4 | 92.6 |
| Redundancy | 4.5 | 4 | 4.5 |
| CC(1/2) | 0.995 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM Bis-Tris pH 5.8 26% (W/V) PEG3350 260 mM Ammonium Sulfate |
