5TXM

STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING DDATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	104
Detector technology	PIXEL
Collection date	2010-09-17
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	90.337, 133.946, 139.399
Unit cell angles	90.00, 97.71, 90.00

Refinement procedure

Resolution	46.046 - 2.700
R-factor	0.1848
Rwork	0.184
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v4i
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.065	0.570
Number of reflections	87934
<I/σ(I)>	12.9
Completeness [%]	97.4	91.1
Redundancy	4.2	3.1
CC(1/2)		0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 8000, AMMONIUM SULFATE, MGCL2, GLYCEROL, SUCROSE,